HSV-1 virus Virios Therapeutics, Inc. (Nasdaq: “VIRI”) New Scientific Paradigm Exploring Herpes Simplex 1 (HSV-1) Virus Activation as Potential Underlying Cause of Fibromyalgia (FM) and Other Chronic Conditions Investor Update Q1 2021 1

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Virios Therapeutics Summary 3 Virios Team and Board of Directors Have Deep Experience in Developing and Commercializing FM and Antiviral Medicines The FM Market is Large, but Dissatisfied – Significant Commercial Potential for Differentiated New FM Treatment Unique IMC-1 Antiviral Combination (famciclovir & celecoxib) Garnered FDA Fastrack Review Designation, Patent Coverage Until 2033 Positive IMC-1 P2b Results in Q2 ‘22 Could Transform FM Patient Care Our Lead Asset, Oral IMC-1, Demonstrated Significant FM Pain Reduction and Tolerability Benefits in Phase 2a Clinical Trial

Proven Leadership Team with Experience in Fibromyalgia (FM) Development and Commercialization 4 Pharma Brand Development & Commercialization Experience Includes Management of: Greg Duncan Chairman & CEO R. Michael Gendreau MD, PhD CMO Ralph Grosswald VP of Operations Angela Walsh SVP of Finance E X E C U T I V E T E A M

Fibromyalgia Disease Overview 5 • FM is a Chronic Disease that Affects up to 8% of the US Population • Hallmark Characteristics are Widespread Chronic Pain and Severe Fatigue • Symptoms Present for ≥ 3 Months • Other Symptoms May Include GI, Sleep, Mood Disorder and Headache Devastating Impact • Patients with FM > 3x Risk of Committing Suicide v. General Population • High Healthcare Utilization and Significant Disability • Estimates Suggest as Many as 40% of FM Patients are Treated with Opioids - Opioid-treated Patients have Worse Outcomes Across Multiple Assessment Domains Disease Characteristics Sources: The Hidden Impact of Musculoskeletal Disorders on Americans, 4th edition; Berger et al Clin Pract 2007; White et al J Occup Environ Med 2008; Wolfe et al Arthritis Care & Res 2014; Fitzcharles et al Am J Med 2011; Robinson et al Pain Medicine 2012; Peng et al Clin J Pain 2015

0 50 100 150 200 250 300 Asia Africa Europe Latin America 3% Prevalence 6% Prevalence 10.0M 3.6M 2.0M Total FM Patients Diagnosed Patients Treated Patients The Fibromyalgia Market is Large and Poised for Growth if Better Therapeutic Options Emerge 6 Worldwide FM Prevalence Ranges From 3%-6% of the Population Significant US FM Market Growth Potential Still Exists 36% Diagnostic Rate 20% Treatment Rate 139M 279M 40M 80M 22M 44M 20M 39M Est. 221M - 447M FM Patients Across the Globe Source: National Fibromyalgia and Chronic Pain Association 2021; Vincent, A et al Arthritis Care Research 2013; Robinson et al Pain Medicine, 2012

Polypharmacy and Utilization of Unapproved Therapies Demonstrate Significant Unmet Need 7 Approved FM Medications Not Approved for FM 75% 25% 26.8% 24.5% 8.9% 0% 10% 20% 30% 40% Cymbalta (duloxetine) Lyrica (pregablin) Savella Approved Medicines • 2.0M Rx Treated FM Patients • Avg 2.6 Rx per Patient Share of US Treated FM Patients by Therapy Source: Robinson et al, Pain Medicine, 2012, . Lumleian primary research for Virios Therapeutics, Inc. Only 15% of Prescribers Reported Satisfaction with Existing Treatment

Discovery Implicates Dormant HSV-1 Reactivation Triggers Immune Response and Manifestation of Fibromyalgia 8 The World Health Organization Estimates That 3.7 Billion People Under the Age of 50 – or 67% of the Population are infected with Herpes Simplex Virus Type 1 (HSV-1), a Life-long Infection Source: P.A. Bond, Medical Hypotheses, 1993,; R. A Vere Hodge and Y.-G. Cheng, Antiviral Chemistry & Chemotherapy, 1993; Liu Y, et al, Scientific World Journal, 2014; Higaki S, et al Current Eye Research, 2009; Francisco Javier Ibañez et al, Frontiers in Microbiology, 2018

IMC-1’s Synergistic Antiviral Mechanism Serves as Basis for Proposed Fibromyalgia Treatment Effect 9 IMC-1 Inhibits HSV-1 Virus, FM Pain Reduced Source: P.A. Bond, Medical Hypotheses, 1993,; R. A Vere Hodge and Y.-G. Cheng, Antiviral Chemistry & Chemotherapy, 1993; Liu Y, et al, Scientific World Journal, 2014; Higaki S, et al Current Eye Research, 2009; Francisco Javier Ibañez et al, Frontiers in Microbiology, 2018

10 Viral GI Tissue Study: Patient population: • 30 Patients who Presented with Both FM and a Chronic GI Disorder • 15 Control Patients, No FM or GI Disorder GI Biopsies were Evaluated for Herpesvirus Infection: • Analysis for ICP8 Viral Protein • ICP8 Only Present During Active HSV-1 Infection • PCR was Used to Detect Herpesvirus DNA IMC-1 Target Antiviral Mechanism Corroborated by GI Biopsy Research Executed with the University of Alabama 0 5 10 15 20 25 30 FM+GI Control Number of Patients HSV-1 No HSV-1 Active HSV-1 Infection (ICP8) (Fisher’s Exact Test) P=<0.0001 Source: Data on file, Virios Therapeutics, Inc.

Week 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 IMC-1 N = 69 Placebo N = 74 11 Design Summary: • Randomized, Double-blind, Multi-center, Placebo-controlled • IMC-1 (famciclovir + celecoxib) vs Placebo, Dosed Twice Daily • Famciclovir Dose Not Optimized • Diagnosis of Fibromyalgia Using 2010 American College of Rheumatology, Assessments at Weeks 6, 12 and 16 • Stop Taking NSAIDs at Randomization • 7-day Washout of FM Drugs and Opioids IMC-1: Completed Phase 2a Clinical Proof of Concept Trial Primary Endpoints Reduction in Pain Key Secondary Endpoints PGIC, FIQ-R Domains, 30% & 50% pain responder analyses Week 6 Week 12 Week 16 Received study drug treatment for a total of 16 weeks Baseline Patients 18–70 Years Old Randomized 1:1 (N = 143)

IMC-1 Demonstrated Statistically Significant Reduction in Pain in Phase 2a Clinical Trial 12 p = 0.031 p = 0.001 Source: Pridgen et al, Journal of Pain Research, 2017

IMC-1 Treatment Resulted in Consistent Treatment Effects at 16 Weeks Across Spectrum of Fibromyalgia Endpoints 13 Secondary Endpoints P Value PROMIS (NIH) Fatigue Assessment p=0.001 PGIC - Patient’s Global Impression of Change P=0.040 FIQ-R- Revised Fibromyalgia Impact Questionnaire Total Score p=0.002 FIQ-R – Functional Domain p=0.004 FIQ-R – Symptoms Domain p=0.004 FIQ-R – Overall Impact Domain p=0.003 Pain Responder Analysis – 50% Pain Reduction • 24 Hour Recall NRS • 7 Day Recall NRS p=0.009 p=0.001 Pain Responder Analysis – 30% Pain Reduction 24 Hour Recall NRS @ week 16 7 Day Recall NRS @ week 16 p=0.052 p=0.012 Use of Rescue Medication p=0.037 Source: Pridgen et al, Journal of Pain Research, 2017

IMC-1 Had a Lower Discontinuation Rate Versus Placebo in Fibromyalgia Phase 2a Study 14 Category Placebo IMC-1 IMC-1 Difference Randomized 74 69 Completed 16 weeks on study drug 45 (60.8%) 57 (82.6%) 22% Discontinuation reasons: Adverse event 12 (16.2%) 4 (5.8%) 2.8X reduction Therapeutic failure 12 (16.2%) 5 (7.2%) 2.3X reduction Other 5 (6.8%) 3 (4.4%) 1.5X reduction (p=0.012) Source: Pridgen et al, Journal of Pain Research, 2017

IMC-1 Phase 2b Design to be Conducted Using Optimized IMC-1 Dosage 15 Secondary Endpoints: Change in fatigue, sleep disturbance, global health status, and patient functionality FM Patients 2016 ACR criteria Age 18 - 65 Sample Size 460 (~230/arm) Treatments IMC-1 vs Placebo Daily Assessments 16 Weeks Design: Randomized, double-blind, multi-center, placebo-controlled trial Primary Endpoint: Reduction in pain 2021 2022 Q1 Q2 Q3 Q4 Q1 Q2 Manufacture Clin Supply Study Start-up Enrollment Study Duration P2b Topline Results Chronic Toxicology Study Anticipated to Run in Parallel with the P2b Clinical Trial

IMC-1 Pipeline Potential Extends to Other Functional Somatic Syndromes 16 • IMC-1 Statistically Significant P2a FM data • FDA Fast Track Review Designation, Progressing to P2b FIBROMYALGIA • Univ. of AL GI Biopsy Data Confirm Active HSV-1 in IBS • ROME IV IBS Assessment Criteria Places Increasing Focus on Pain IRRITABLE BOWEL SYNDROME • Viral infections identified as possible triggers of CFS/ME • Fatigue most common symptom beyond 2 weeks after COVID-19 onset • IMC-1 Statistically Significant Reduction in fatigue in FM P2a CHRONIC FATIGUE SYNDROME/ MYALGIC ENCEPHALITIS Source: WL Pridgen, MD et al, Journal of Pain Research, 2017; Virios Therapeutics Data on File, 2021; Santa Rosa et al, Journal of Translational Medicine, 2018; Rita Rubin, PhD, MA JAMA 2020

A New Treatment for Fibromyalgia Could Have Significant Commercial Potential in the US Market Alone 17 Existing Market of 3.6M Dx Full Market Potential: 10.0M Total Projected US FM Community 500K 25% Patient Share of Treated FM Population @ 500K Unique Patients Lyrica @ $16/day in 2019, Est. 60% Compliance Rate, Annual FM Sales Est. @ $1.75B FM Costs US $12-14B Annually, 1-2% Lost Productivity in US Source: National Fibromyalgia and Chronic Pain Association, 2021; Evaluate Pharma, 2019; Robinson et al Pain Medicine 2012, Vincent et al Arthritis Care Research, 2013; Redbook, 2019

Capital Table as of February 28th 2021 18 Listing/Symbol Nasdaq/VIRI Common Shares Outstanding 8,330,390 Underwriters Warrants* (Exercisable at $12.50/share) 172,500 Stock Options (Employees, Directors & Officers)** 874,397 Fully Diluted Shares Outstanding 9,377,287 Management Ownership on a Fully Diluted Basis 18.4% *Become Exercisable on December 16, 2021 **Weighted Avg. Exercise Price of $9.80/share

Virios Therapeutics Summary 19 Virios Team and Board of Directors Have Deep Experience in Developing and Commercializing FM and Antiviral Medicines The FM Market is Large, but Dissatisfied – Significant Commercial Potential for Differentiated New FM Treatment Unique IMC-1 Antiviral Combination (famciclovir & celecoxib) Garnered FDA Fastrack Review Designation, Patent Coverage Until 2033 Positive IMC-1 P2b Results in Q2 ‘22 Could Transform FM Patient Care Our Lead Asset, Oral IMC-1, Demonstrated Significant FM Pain Reduction and Tolerability Benefits in Phase 2a Clinical Trial